New article online: The effects of continuously acoustical stress on cortisol in milkfish

The effects of continuously acoustical stress on cortisol in milkfish (Chanos Chanos)

General and Comparative Endocrinology (2017)

Chih An Wei, Yi-Ta Shao*
Institute of Marine Biology, National Taiwan Ocean University

Tzu-Hao Lin
Research Center for Information Technology Innovation, Academia Sinica

Ruo Dong Chen
Institute of Cellular and Organismic Biology, Academia Sinica

Yung-Che Tseng
Marine Research Station, Institute of Cellular and Organismic Biology, Academia Sinica

Strong underwater acoustic noise has been known that may cause hearing loss and actual stress in teleost. However, the long-term physiological effects of relatively quiet but continuously noise on fish were less understood. In present study, milkfish, Chanos chanos, were exposed to the simulated-wind farm noise either quiet (109 dB re 1 μPa / 125.4 Hz; approx. 10-100m distant from the wind farm) or noisy (138 dB re 1 μPa / 125.4 Hz; nearby the wind farm) conditions for 24 hr, 3 days and 1 week. Comparing to the control group (80 dB re 1 μPa / 125.4 Hz), the fish exposed to noisy conditions had higher plasma cortisol levels in the first 24 hr. However, the cortisol levels of 24 hr spot returned to the resting levels quickly. The fish exposed under noisy condition had significantly higher head kidney star (steroidogenic acute regulatory) and hsd11b2 (11-β-hydroxysteroid dehydrogenase 2) mRNA levels at the following treatment time points. In addition, noise exposure did not change hypothalamus crh (Corticotropin-releasing hormone) mRNA levels in this experiment. The results implied that the weak but continuously noise was a potential stressor to fish, but the impacts may be various depending on the sound levels and exposure time. Furthermore, this study showed that the continuous noise may up-regulate the genes that are related to cortisol synthesis and possibly make the fish more sensitive to ambient stressors, which may influence the energy allocation appearance in long-term exposures.

New article online: Improving biodiversity assessment via unsupervised separation of biological sounds from long-duration recordings

Our new article has been published on Scientific Reports! In this article, we introduce a novel machine learning tool, the periodicity coded nonnegative matrix factorization (PC-NMF). The PC-NMF can separate biological sounds from a noisy long-term spectrogram in an unsupervised approach, therefore, it is a great tool for evaluating the dynamics of soundscape and facilitating the soundscape-based biodiversity assessment.

You can download the MATLAB codes of PC-NMF and test data in the supplementary dataset of our article.

Improving biodiversity assessment via unsupervised separation of biological sounds from long-duration recordings

Scientific Reports 7, 4547 (2017) doi:10.1038/s41598-017-04790-7

Tzu-Hao Lin, Yu Tsao
Research Center for Information Technology Innovation, Academia Sinica, Taipei, Taiwan (R.O.C.)

Shih-Hua Fang
Department of Electrical Engineering, Yuan Ze University, Taoyuan, Taiwan (R.O.C.)

Investigating the dynamics of biodiversity via passive acoustic monitoring is a challenging task, owing to the difficulty of identifying different animal vocalizations. Several indices have been proposed to measure acoustic complexity and to predict biodiversity. Although these indices perform well under low-noise conditions, they may be biased when environmental and anthropogenic noises are involved. In this paper, we propose a periodicity coded non-negative matrix factorization (PC-NMF) for separating different sound sources from a spectrogram of long-term recordings. The PC-NMF first decomposes a spectrogram into two matrices: spectral basis matrix and encoding matrix. Next, on the basis of the periodicity of the encoding information, the spectral bases belonging to the same source are grouped together. Finally, distinct sources are reconstructed on the basis of the cluster of the basis matrix and the corresponding encoding information, and the noise components are then removed to facilitate more accurate monitoring of biological sounds. Our results show that the PC-NMF precisely enhances biological choruses, effectively suppressing environmental and anthropogenic noises in marine and terrestrial recordings without a need for training data. The results may improve behaviour assessment of calling animals and facilitate the investigation of the interactions between different sound sources within an ecosystem.






講座地點:清華大學圖書館 1樓清華沙龍(新竹市光復路二段101號)


對此講座有興趣的朋友,也歡迎閱讀 用「聲物」識生物 以自動錄音聆聽自然谷之聲 這篇文章,在文章裡面,我們簡單的分享了如何應用自動錄音機探索自然谷的豐富生物多樣性,也可以到我們所製作的互動式網頁中,一探自然谷的各種聲物!


2017/5/4-5 @ 國立中山大學


中央研究院 資訊科技創新研究中心

被動式聲學監測已被廣泛應用在海洋環境與生態研究中,長期錄音中的各種環境音與動物音增加了我們對海洋生態環境的了解,許多研究也深入探討人為噪音對海洋生態的影響。然而,過去針對海洋聲景的分析大多著重噪音的時頻譜特性,並透過設定規則的偵測器尋找海洋動物的聲音。但海洋聲景受到地形、氣候、生物群聚與人為活動的高度影響,時頻譜分析可能無法有效描述同時出現的多種聲源,偵測器效能也隨著噪音變動而改變。為了有效分離海洋聲景中的各種聲源,本研究應用非負矩陣分解法 (non-negative matrix factorization) 及其變形方法分析長期時頻譜圖,將輸入資料拆解為特徵矩陣與編碼矩陣。雖然單層的非負矩陣分解法在多次疊代後,能夠在特徵矩陣與編碼矩陣約略學習到各種聲源的頻譜特徵與時域上的強度,但仍難以分離重疊的多種聲源。本研究將多層學習器分別預訓練後堆疊成深度學習架構,並在各層之間逐漸減少特徵矩陣之基底數量,藉由最末層回傳後之重建資料和輸入資料的誤差,在多次疊代中自行修正各層模型參數以達到最佳的聲源分離成果。本研究針對各地具有不同環境噪音特性的海洋聲景進行分析,結果顯示在不需要辨識樣本與資料標籤的情況下,深度學習能夠有效分離海洋中的各種主要聲源:魚群鳴唱、槍蝦脈衝聲、船隻噪音與環境音。學習到的特徵矩陣也能夠作為辨識樣本,透過半監督式學習分析大量的線上資料。透過深度學習分離聲源,未來將能夠更有效評估海洋聲景的複雜結構,並藉此探討海洋環境與生態的變動,以及人為開發的影響。

International Symposium on Grids & Clouds 2017

2017/3/5-10 @ Academia Sinica, Taipei, Taiwan

Listening to the ecosystem: the integration of machine learning and a long-term soundscape monitoring network

Tzu-Hao Lin, Yu Tsao
Research Center for Information Technology Innovation, Academia Sinica

Yu-Huang Wang
Taiwan Biodiversity Information Facility, Biodiversity Research Center, Academia Sinica

Han-Wei Yen
Academia Sinica Grid Computing

Information on the variability of environment and biodiversity is essential for conservation management. In recent years, soundscape monitoring has been proposed as a new approach to assess the dynamics of biodiversity. Soundscape is the collection of biological sound, environmental sound, and anthropogenic noise, which provide us the essential information regarding the nature environment, behavior of calling animals, and human activities. The recent developments of recording networks facilitate the field surveys in remote forests and deep marine environments. However, analysis of big acoustic data is still a challenging task due to the lack of sufficient database to recognize various animal vocalizations. Therefore, we have developed three tools for analyzing and visualizing soundscape data: (1) long-term spectrogram viewer, (2) biological chorus detector, (3) soundscape event classifier. The long-term spectrogram viewer helps users to visualize weeks or months of recordings and evaluate the dynamics of soundscape. The biological chorus detector can automatically recognize the biological chorus without any sound template. We can separate the biological chorus and non-biological noise from a long-term spectrogram and unsupervised identify various biological events by using the soundscape event classifier. We have applied these tools on terrestrial and marine recordings collected in Taiwan to investigate the variability of environment and biodiversity. In the future, we will integrate these tools with the Asian Soundscape monitoring network. Through the open data of soundscape, we hope to provide ecological researcher and citizens an interactive platform to study the dynamics of ecosystem and the interactions among acoustic environment, biodiversity, and human activities.

5th Joint Meeting of the Acoustical Society of America and Acoustical Society of Japan

2016/11/28-12/2 @ Honolulu, USA

Acoustic response of Indo-Pacific humpback dolphins to the variability of marine soundscape

Tzu-Hao Lin, Yu Tsao
Research Center for Information Technology Innovation, Academia Sinica

Shih-Hau Fang
Department of Electrical Engineering, Yuan Ze University

Chih-Kai Yang, Lien-Siang Chou
Institute of Ecology and Evolutionary Biology, National Taiwan University

Marine mammals can adjust their vocal behaviors when they encounter anthropogenic noise. The acoustic divergence among different populations has also been considered as the effect of ambient noise. The recent studies discover that the marine soundscape is highly dynamic; however, it remains unclear how marine mammals alter their vocal behaviors under various acoustic environments. In this study, autonomous sound recorders were deployed in western Taiwan waters between 2012 and 2015. Soundscape scenes were unsupervised classified according to acoustic features measured in each 5 min interval. Non-negative matrix factorization was used to separate different scenes and to inverse the temporal occurrence of each soundscape scene. Echolocation clicks and whistles of Indo-Pacific humpback dolphins, which represent the only marine mammal species occurred in the study area, were automatically detected and analyzed. The preliminary result indicates the soundscape scenes dominated by biological sounds are correlated with the acoustic detection rate of humpback dolphins. Besides, the dolphin whistles are much complex when the prey associated scene is prominent in the local soundscape. In the future, the soundscape information may be used to predict the occurrence and habitat use of marine mammals.


2017/1/23-24 @ 高雄中山大學